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US 2022/0033832 A1Published application
Modified aox1 promoter variants
AssigneeOrta Dogu Teknik Universitesi
InventorsPinar CALIK, Burcu GUNDUZ ERGUN
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AssigneeOrta Dogu Teknik Universitesi
InventorsPinar CALIK, Burcu GUNDUZ ERGUN
Estimating the term…
Pichia pastoris alcohol oxidase 1 (AOX1) promoter variants include at least one of the specified modifications on wild-type Pichia pastoris AOX1 promoter (SEQ ID NO: 1). The modifications include the following: a) integration of a Cat8 transcription factor binding site (TFBS), particularly integration of SEQ ID NO: 6 or SEQ ID NO: 7 or other gene sequences that show at least 80% similarity with these sequences, at any position within nucleotides 94 to 110, 141 to 160, 312 to 330, 355 to 380, 501 to 521; 640 to 658, 674 to 693, and 1 to 840; b) integration of Aca1 or Aca2 TFBS particularly integration of SEQ ID NO: 8 or other gene sequences showing at least 80% similarity with this sequence at any position between the nucleotides 1 to 840; c) mutations specified with SEQ ID NO: 2 within nucleotides 94 to 693 and combinations thereof.
A Pichia pastoris alcohol oxidase 1 (AOX1) promoter variant, comprising at least one mutation within nucleotides 1 to 840 of a nucleic acid sequence of a wild-type P. pastoris AOX1 promoter, wherein the nucleic acid sequence of the wild type P. pastoris AOX1 promoter is set forth by SEQ ID NO:
(canceled)
Dependencies are inferred from the claim text.