Method and kit for construction of rna library

A method for preparing an RNA library, comprising the following steps:

• (i) (a) extracting RNA and performing fragmentation;
• or (b) extracting RNA;
• (ii) adding a tail to 3′ end;
• (iii) ligating an adaptor to 5′ end and enriching a target RNA fragment;
• (iv) performing reverse transcription and PCR amplification to obtain a library solution.

A kit for preparing an RNA library, comprising

• (1) a component A, a component B, a component C, a component D, a component E, and a component F;
• (2) a component A, a component G, a component D, a component E, and a component F;
• (3) a component A, a component H, a component B, a component C, a component D, a component E, and a component F;
• (4) a component A, a component H, a component G, a component D, a component E, and a component F;
• (5) a component I, a component B, a component C, a component D, a component E, and a component F;
• or,
• (6) a component I, a component G, a component D, a component E, and a component F;
• wherein:
• Component A is a reagent or a combination of reagents for adding a tail to the 3′ end of an RNA molecule;
• Component B is a reagent or a combination of reagents for ligating an adaptor to the 5′ end of an RNA molecule;
• Component C is a DNA probe mixture;
• the DNA probe mixture is composed of several DNA probes that are reverse complementary to an RNA that is expected to be removed;
• Component D is a reagent or a combination of reagents for removing RNA from the hybrid and removing DNA;
• Component E is a reagent or a combination of reagents for reverse transcription;
• Component F is a reagent or a combination of reagents for PCR;
• Component G is a reagent or a combination of reagents for ligating an adaptor to the 5′ end of an RNA molecule and for hybridizing with a DNA probe;
• Component H is a reagent or a combination of reagents for modifying an RNA fragment to have a phosphate group at its 5′ end and a hydroxyl group at its 3′ end;
• Component I is a reagent or a combination of reagents for tailing and modifying the 3′ end of an RNA molecule.