US 2022/0033803 A1 · METHOD OF EXTRACTING MIRNA AND METHOD OF ANALYZING MIRNA

Abstract

The present invention provides a novel miRNA extraction method and a method for analyzing miRNA extracted by using said miRNA extraction method. According to the present invention, provided is, for example, a method for extracting miRNA from extracellular vesicles in a sample solution, by using a device capable of capturing extracellular vesicles, the miRNA extraction method comprising: an extracellular vesicle capturing step for capturing extracellular vesicles in a sample solution onto a device by bringing the sample solution and the device in contact with each other; and a miRNA extraction step for homogenizing the extracellular vesicles by bringing the device having captured the extracellular vesicles in contact with a homogenization liquid for extracellular vesicles to extract miRNA from the extracellular vesicle into the homogenization liquid.

Claims10

Dependent claims

A method of extracting miRNA from extracellular vesicles in a sample solution using a device capable of capturing extracellular vesicles, the method comprising:

an extracellular vesicle capture comprising bringing the sample solution into contact with the device, to capture an extracellular vesicle in the device;
and
a miRNA extraction comprising bringing the device that captured the extracellular vesicle with a disruption solution of extracellular vesicles, to disrupt the extracellular vesicle and extract miRNA from the extracellular vesicle into the disruption solution.

Dependencies are inferred from the claim text.

Facts

Application

17/289,611Filed 30 Oct 2019

Priority

JP 2018-20352730 Oct 2018

Published

3 Feb 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 15/1006
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
C12Q 1/6876
Involving nucleic acids: nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
G01N 33/48
Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00: biological material, e.g. blood, urine ;…
G01N 33/50
Biological material, e.g. blood, urine ; Haemocytometers: chemical analysis of biological material, e.g. blood, urine; Testing involving…

Method of extracting mirna and method of analyzing mirna

Life of this patent

Method of extracting mirna and method of analyzing mirna

Life of this patent

Abstract

Claims10