Method and apparatus for encoding cellular spatial position information

A method comprising:

• adding, to each of a plurality of different microsamples from a biological specimen, a marker comprising spatial information that encodes the original spatial position of the microsample within the biological specimen.

-33. (canceled)

The method of claim Error! Reference source not found., wherein the oligonucleotide further comprises a poly T tail, and the method comprises capturing mRNA molecules from the microsamples having a poly T tail;

• and reverse transcribing the mRNA molecules to produce cDNA molecules comprising the barcode where the barcode provides the spatial information.

The method of claim Error! Reference source not found.7, wherein the oligonucleotide further comprises a capture sequence complementary to a target sequence, and the method comprises capturing DNA molecules from the microsample having the target sequence;

• and extending the oligonucleotide to produce a nucleic acid molecule having a copy of the target sequence and comprising the barcode, wherein the barcode provides the spatial information.

-38. (canceled)

-42. (canceled)

-54. (canceled)

A method comprising entraining in a fluidic stream a plurality of microsamples from a biological specimen, wherein the microsamples are contained in spatially separated microdrops in the fluidic stream and positioned in an order based on their original spatial position within the biological specimen.