Engineered biosynthetic pathways for production of 1,5-diaminopentane by fermentation
AssigneeZymergen Inc.
InventorsAaron Miller, Zhihao Wang, Jeffrey Mellin, Murtaza Shabbir Hussain, Steven M. Edgar
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Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of 1,5-diaminopentane and provides novel engineered microbial cells and cultures, as well as related 1,5-diaminopentane production methods.
Claims57
An engineered microbial cell that expresses a non-native lysine decarboxylase, wherein the engineered microbial cell produces 1,5-diaminopentane.
An engineered microbial cell, wherein the engineered microbial cell comprises means for expressing a non-native lysine decarboxylase, and wherein the engineered microbial cell produces 1,5-diaminopentane.
A method for preparing 1,5-diaminopentane using microbial cells engineered to produce 1,5-diaminopentane, the method comprising:
- (a) expressing a non-native lysine decarboxylase in microbial cells;
- (b) cultivating the microbial cells in a suitable culture medium under conditions that permit the microbial cells to produce 1,5-diaminopentane, wherein the 1,5-diaminopentane is released into the culture medium;
- and
- (c) isolating 1,5-diaminopentane from the culture medium.
Dependencies are inferred from the claim text.