Methods for ex vivo expansion of natural killer cells and use thereof
AssigneeBoard of Regents, The University of Texas System
InventorsElizabeth Shpall, Katy Rezvani
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Abstract
Provided herein are ex vivo methods for the expansion of cord blood-derived natural killer cells and methods of their use. Examples of embodiments include stimulating mononuclear cells from cord blood in the presence of antigen presenting cells (APCs) and IL-2 and re-stimulating the cells with APCs to produce expanded NK cells. In specific embodiments, the method does not utilize human leukocyte antigen (HLA) matching.
Claims78
An ex vivo method for the expansion of natural killer (NK) cells comprising:
- (a) obtaining a starting population of mononuclear cells (MNCs) from cord blood;
- (b) stimulating the MNCs in the presence of antigen presenting cells (APCs) and IL-2;
- and
- (c) re-stimulating the cells with APCs to produce expanded NK cells, wherein the method is performed in a bioreactor and is good manufacturing practice (GMP) compliant.
The method of claim 75, wherein the at least a second therapeutic agent comprises chemotherapy, immunotherapy, surgery, radiotherapy, or biotherapy.
Dependencies are inferred from the claim text.