Compositions and methods for cytotoxic cd4+ t cells
OwnerDavid OH, Lawrence FONG +3inventors
InventorsDavid OH, Lawrence FONG, Serena KWEK MACPHEE, Chun Jimmie YE, Chien-Chun Steven PAI
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Abstract
Provided herein, inter alia, are compositions and methods for treating bladder cancer, including CD4+T cells that can be cultured ex vivo to generate a population of cytotoxic CD4+T cells capable of killing bladder cancer tumor cells. Pharmaceutical compositions containing such a cytotoxic CD4+T cell population, as well as methods for treating an individual having or suspected of having bladder cancer are also provided.
Claims27
An ex vivo population of CD4+ T cells, wherein the CD4+ T cells express one or more markers selected from the group consisting of GZMA, GZMB, GZMH, GZMK, KLRB1, KLRD1, GNLY, NKG7, CCL4, CCL5, LTB, CXCR4, CXCR6, PRF1, KLRG1, LAG3, CXCL13, and combinations of any thereof, and wherein the CD4+ T cells have cytolytic capabilities.
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A method for producing an ex vivo expanded population of CD4+ T cells with cytolytic capabilities, the method comprising:
- (a) separating CD4+ T cells from a biological sample containing a mixture of different types of immune cells;
- (b) culturing the separated CD4+ T cells in media containing IL-2 in an amount sufficient to promote the expansion of CD4+ T cells;
- (c) splitting the cultured CD4+ T cells to promote the enrichment of CD4+ T cells thus producing an ex vivo expanded population of CD4+ T cells with cytolytic capabilities.
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A method for providing a cell therapy or for treating an individual having or suspected of having bladder cancer, the method comprising administering to the individual a composition comprising an effective amount of a population of cytolytic CD4+ T cells.
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Dependencies are inferred from the claim text.