Back to results
US 2022/0033764 A1Published application
Engineered microorganisms and methods of making and using same
AssigneeVir Biotechnology, Inc.
InventorsGautam DANTAS, Suryang KWAK, Herbert VIRGIN
Life of this patent
Estimating the term…
Abstract
The present disclosure provides for engineered microorganisms and methods of making and using same. The engineered microorganisms as described herein can have a surface display and can be useful as therapeutic agents (e.g., sponges) and biosensors.
Claims73
An engineered microorganism comprising:
- at least one binding agent displayed on a surface of the engineered microorganism, wherein the binding agent has a binding affinity to a target microorganism.
A method of constructing an engineered microorganism comprising:
- providing a microorganism;
- providing a display cassette;
- and
- integrating the display cassette into an intergenic site of the microorganism genome;
- wherein,
- the display cassette comprises a sequence for expression of a binding agent;
- a promoter capable of expressing the binding agent;
- a terminator;
- an anchor;
- and a secretion signal peptide.
7 more elements
A method of constructing an engineered microorganism comprising:
- providing a microorganism;
- providing a display cassette;
- and
- integrating the display cassette into an intergenic site of the microorganism genome;
- wherein,
- the display cassette comprises a sequence for expression of a binding agent;
- a constitutive promoter;
- a terminator;
- a stem comprising a transmembrane domain of a microorganism cell wall protein;
- a secretion signal peptide;
- and an intracellular enzyme-cleavable-binding fragment operably linked to a transactivator.
8 more elements
Dependencies are inferred from the claim text.