US 2022/0033738 A1 · POLYPEPTIDES HAVING NUCLEASE ACTIVITY

Abstract

The present invention relates to polypeptides having nuclease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and recombinant host cells comprising the polynucleotides as well as methods of producing, recovering and using the polypeptides. The invention further relates to cleaning compositions comprising one or more of the polypeptides having nuclease activity, as well as a cleaning method and use of the polypeptides.

Claims20

Dependent claims

A cleaning composition comprising:

(a) at least 0.001 ppm of a polypeptide have nuclease activity, wherein the polypeptide comprises one or more of the motifs [HQ][FILVY]X[GAQS]DX[HTGSA][QVM]P[LFM]H (SEQ ID NO:
102), G[GA]NX[VILFY]X[VLM] (SEQ ID NO:
103), [SADN]R[GS]H (SEQ ID NO:
104), P[LM]H[VA][GA] (SEQ ID NO:
112), PLH[DN]E (SEQ ID NO:
113), [YV][DN]RGH (SEQ ID NO:
114), YDRGHQ[AV] (SEQ ID NO:
115), [DNA]R[GSC]H[LI] (SEQ ID NO:
116) and/or [RIENLG][YF][RHN]V (SEQ ID NO:
117);
wherein the polypeptide belongs to the Pfam family PF02265 (S1-P1_nuclease), PF01223 (Endonuclease_NS) or PF13930 (Endonuclea_NS_2);
and
(b) one or more surfactants.

10 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/276,436Filed 3 Oct 2019

Priority

CN PCT/CN2018/1092434 Oct 2018

Published

3 Feb 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12Y 301/21001
Endodeoxyribonucleases producing 5'-phosphomonoesters: deoxyribonuclease I
C11D 3/386
Products with no well-defined composition , e.g. natural products: preparations containing enzymes , e.g. protease or amylase
C11D 3/38636
Preparations containing enzymes , e.g. protease or amylase: containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase

Polypeptides having nuclease activity

Life of this patent

Polypeptides having nuclease activity

Life of this patent

Abstract

Claims20