Methods of modulating antibody-dependent cell-mediated cytotoxicity

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A method for engineering a specific target Antibody Dependent Cellular Cytotoxicity (ADCC) activity of a glycosylated and afucosylated IgG1 antibody composition comprising:

• (1) determining the ADCC activity of a glycosylated and afucosylated IgG1 antibody composition;
• (2) determining a target ADCC activity;
• and (3) increasing or decreasing the ADCC activity of the glycosylated and afucosylated IgG1 antibody composition by increasing or decreasing the amount of terminal β-galactose in the glycan species at the consensus glycosylation site, wherein the ADCC activity of the glycosylated and afucosylated IgG1 antibody composition after increasing or decreasing the amount of terminal β-galactose is the same as the target ADCC activity or within about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45% or about 50% of the target ADCC activity or within about 1% to about 50% of the target ADCC activity.

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