Antibody for pure isolation of vascular endothelial cells and preparation method thereof
AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsYoung Sup YOON, Shin Jeong LEE, Dong Chan SON
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Abstract
The present specification provides an antibody for pure isolation of vascular endothelial cells and a preparation method thereof, the antibody comprising: a heavy chain variable domain and a light chain variable domain which specifically bind to an extracellular matrix domain of PECAM1 having an amino acid sequence of SEQ ID NO: 1; or a heavy chain variable domain and a light chain variable domain which specifically bind to an extracellular matrix domain of CDH5 having an amino acid sequence of SEQ ID NO: 2.
Claims19
An antibody for purifying a vascular endothelial cell, the antibody containing a heavy-chain variable domain and a light-chain variable domain specifically binding to an extracellular matrix domain of PECAM1 having an amino acid sequence represented by SEQ ID NO:
- 1 or CDH5 having an amino acid sequence represented by SEQ ID NO:
- 2.
A method for producing an antibody for purifying a vascular endothelial cell, the method comprising:
- injecting a protein containing an extracellular matrix domain of PECAM1 having an amino acid sequence represented by SEQ ID NO:
- 1 into an antibody producing subject so as to produce an antibody against the PECAM1;
- determining a positive clone reacting with an antigen of the PECAM1 from the antibody producing subject;
- and
- separating the antibody against the PECAM1 from the positive clone.
A method for producing an antibody for purifying a vascular endothelial cell, the method comprising:
- injecting a protein containing an extracellular matrix domain of CDH5 having an amino acid sequence represented by SEQ ID NO:
- 2 into an antibody producing subject so as to produce an antibody against the CDH5;
- determining a positive clone reacting with an antigen of the CDH5 from the antibody producing subject;
- and
- separating the antibody against the CDH5 from the positive clone.
Dependencies are inferred from the claim text.