Antibody against human cardiac troponin I and use thereof

An isolated binding protein comprising an antigen binding domain, wherein the antigen binding domain comprises at least one complementarity determining region selected from the following amino acid sequences, or has at least 80% sequence identity with the complementarity determining region of the following amino acid sequences and has KD≤9.96×10−8 mol/L affinity with a cTnI;

• a complementarity determining region CDR-VH1 is G-Y-X1-F-T-X2-Y-V-X3-H, wherein,
• the X1 is S or T, X2 is I or L, and the X3 is V, L or I;
• a complementarity determining region CDR-VH2 is Y-I-X1-P-Y-X2-D-G-T-X3-Y-N-E-K, wherein,
• the X1 is Q, N or Y, the X2 is I or L, and the X3 is R or K;
• a complementarity determining region CDR-VH3 is R-X1-G-Y-G-X2-Y-G-L-A, wherein
• the X1 is S or T, and the X2 is Q, N or G;
• a complementarity determining region CDR-VL1 is S-X1-G-A-X2-T-T-S-X3-Y-A-N, wherein,
• the X1 is S or T, the X2 is A or V, and the X3 is Q or N;
• a complementarity determining region CDR-VL2 is G-S-X1-N-R-X2-P, wherein,
• the X1 is N or Q, and the X2 is A or V;
• a complementarity determining region CDR-VL3 is A-X1-V-Y-S-N-X2-W, wherein,
• the X1 is I or L, and the X2 is Q, H or N.