Adeno-associated virus antibodies and fragments thereof
AssigneeSarepta Therapeutics, Inc.
InventorsSohrab KHAN, Danielle GRIFFIN, Louise RODINO-KLAPAC
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to an isolated anti-AAV (adeno-associated virus) antibody or an antigen-binding fragment thereof capable of specifically binding an epitope of AAVrh74 capsid protein and uses thereof.
Claims26
1-60. (canceled)
An isolated antibody or antigen-binding fragment thereof that comprises a heavy chain variable region comprising VH CDR1, VH CDR2, and VH CDR3 domains, and a light chain variable region comprising VL CDR1, VL CDR2, and VL CDR3 domains, wherein:
- a. the VH CDR1 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 39, SEQ ID NO:
- 46, SEQ ID NO:
- 52, and SEQ ID NO:
- 58;
- b. the VH CDR2 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 34, SEQ ID NO:
- 40, SEQ ID NO:
- 47, SEQ ID NO:
- 53, and SEQ ID NO:
- 59;
- c. the VH CDR3 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 35, SEQ ID NO:
- 41, SEQ ID NO:
- 48, SEQ ID NO:
- 54, and SEQ ID NO:
- 60;
- d. the VL CDR1 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 36, SEQ ID NO:
- 42, SEQ ID NO:
- 49, SEQ ID NO:
- 55, and SEQ ID NO:
- 61;
- e. the VL CDR2 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 37, SEQ ID NO:
- 43, SEQ ID NO:
- 50, SEQ ID NO:
- 56, and SEQ ID NO:
- 62;
- or
- f. the VL CDR3 domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
- 38, SEQ ID NO:
- 44, SEQ ID NO:
- 51, SEQ ID NO:
- 57, and SEQ ID NO:
- 63.
The isolated antibody or antigen binding fragment thereof of 71, comprising a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:
- 9 and a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO:
- 10.
An isolated polynucleotide comprising a nucleic acid sequence that encodes
- a. a VH CDR1 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 33, SEQ ID NO:
- 39, SEQ ID NO:
- 46, SEQ ID NO:
- 52, and SEQ ID NO:
- 58;
- b. a VH CDR2 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 34, SEQ ID NO:
- 40, SEQ ID NO:
- 47, SEQ ID NO:
- 53, and SEQ ID NO:
- 59;
- c. a VH CDR3 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 35, SEQ ID NO:
- 41, SEQ ID NO:
- 48, SEQ ID NO:
- 54, and SEQ ID NO:
- 60;
- d. a VL CDR1 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 36, SEQ ID NO:
- 42, SEQ ID NO:
- 49, SEQ ID NO:
- 55, and SEQ ID NO:
- 61;
- e. a VL CDR2 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 37, SEQ ID NO:
- 43, SEQ ID NO:
- 50, SEQ ID NO:
- 56, and SEQ ID NO:
- 62;
- or
- f. a VL CDR3 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 38, SEQ ID NO:
- 44, SEQ ID NO:
- 51, SEQ ID NO:
- 57, and SEQ ID NO:
- 63.
Dependencies are inferred from the claim text.