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US 2022/0033471 A1Published application
Methods for producing aflibercept in chemically defined media having reduced aflibercept variants
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Mastrogiacomo, Shunhai Wang, Ning Li
Life of this patent
Estimating the term…
Abstract
The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
Claims24
A polypeptide having an isolated amino acid sequence comprising at least 70% sequence identity over a full length of said isolated amino acid sequence as set forth in the group consisting of SEQ ID NO.:
- 2, SEQ ID NO.:
- 3, SEQ ID NO.:
- 4, SEQ ID NO.:
- 5, SEQ ID NO.:
- 6, SEQ ID NO.:
- 7, SEQ ID NO.:
- 8, SEQ ID NO.:
- 9, SEQ ID NO.:
- 10, SEQ ID NO.:
- 11, SEQ ID NO.:
- 12, SEQ ID NO.:
- 13, SEQ ID NO.:
- 14, SEQ ID NO.:
- 15 and SEQ ID NO.:
- 16, wherein said polypeptide excludes IdeS as set forth in SEQ ID NO.:
- 1.
13 more elements
An isolated nucleic acid molecule encoding a polypeptide with an amino acid sequence as set forth in the group consisting of SEQ ID NO.:
- 2, SEQ ID NO.:
- 3, SEQ ID NO.:
- 4, SEQ ID NO.:
- 5, SEQ ID NO.:
- 6, SEQ ID NO.:
- 7, SEQ ID NO.:
- 8, SEQ ID NO.:
- 9, SEQ ID NO.:
- 10, SEQ ID NO.:
- 11, SEQ ID NO.:
- 12, SEQ ID NO.:
- 13, SEQ ID NO.:
- 14, SEQ ID NO.:
- 15 and SEQ ID NO.:
- 16, wherein said polypeptide excludes IdeS as set forth in SEQ ID NO.:
- 1.
13 more elements
An isolated amino acid comprising a parental amino acid sequence defined by SEQ ID NO.:
- 1 with asparagine residues at one or more positions of 87, 130, 182 or 274 mutated to an amino acid other than asparagine.
Dependencies are inferred from the claim text.