High affinity cell receptor for recognizing afp antigen

A T cell receptor (TCR) having an activity of binding to FMNKFIYEI-HLA A0201 complex, and the T cell receptor comprises a TCR α chain variable domain and a TCR β chain variable domain, the TCR α chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR α chain variable domain are listed as follows,

• CDR1α:
• TSINN
• CDR2α:
• IRSNERE
• CDR3α:
• ATDPRTGANSKLT,
• the TCR β chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR β chain variable domain are listed as follows,
• CDR1β:
• SEHNR
• CDR2β:
• FQNEAQ
• CDR3β:
• ASEGLAYEQY,
• wherein the TCR α chain variable domain contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• S at position 2 of CDR1α
• D or P or A or E
• I at position 3 of CDR1α
• V
• N at position 4 of CDR1α
• S or Y or D
• N at position 5 of CDR1α
• S or K
• R at position 5 of CDR3α
• A or S or V or D or E or Q or T
• T at position 6 of CDR3α
• M or R or L or A or N or S
• G at position 7 of CDR3α
• A or Q or S or E or N
• A at position 8 of CDR3α
• S or G or P or W
• N at position 9 of CDR3α
• S or T
• S at position 10 of CDR3α
• A or N or T or P
• and/or, the TCR β chain variable contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• S at position 1 of CDR1β
• A or Q or G
• E at position 2 of CDR1β
• G or T or R or L
• H at position 3 of CDR1β
• W or R
• N at position 4 of CDR1β
• A or P
• Q at position 2 of CDR2β
• H
• N at position 3 of CDR2β
• G
• E at position 4 of CDR2β
• D
• A at position 5 of CDR2β
• M or L
• Q at position 6 of CDR2β
• E.
• and the α chain variable domain of the TCR comprises an amino acid sequence having at least 90% sequence homology with the amino acid sequence shown in SEQ ID NO:
• 1;
• and/or the β chain variable domain of the TCR comprises an amino acid sequence having at least 90% sequence homology with the amino acid sequence shown in SEQ ID NO:
• 2.