System and method for bioremediation of soil
AssigneeThe Trustees of Columbia University in the City of New York
InventorRaymond SAMBROTTO
Life of this patent
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Abstract
Systems and methods for performing bioremediation using the Geobacillus sp. bacterial strain are provided which are capable of effectively degrading Benzo[a]pyrene (BaP) and efficiently degrading other ring-based organic contaminants. The exemplary bioremediation methods include steps for administering an effective, degrading amount of the bacteria to soil containing excess amounts of BaP and incubating the bacteria administered to the soil at a given temperature and for a duration that are suitable for promoting incubation and reducing and the concentration of BaP below a maximum concentration, as might be specified by a regulatory body.
Claims17
A method for bioremediation of soil, sediment or wastewater containing benzo[a]pyrene (BaP), the method comprising the steps of:
- administering Geobacillus midousuji, or a mutant derived therefrom, to the soil, sediment or wastewater to be bioremediated;
- and,
- incubating the Geobacillus midousuji in the soil, sediment or wastewater at a temperature ranging from about 40 to about 70° C. and a humidity ranging from about 80% to about 100% for a period of time ranging from about 1 hour to about 20 days.
A kit comprising:
- Geobacillus midousuji or a mutant derived therefrom;
- printed matter with instructions for activating the Geobacillus midousuji into log phase growth before incubation with soil, sediment or wastewater by incubation of the Geobacillus midousuji under aerobic conditions at a temperature ranging from about 60° to about 65° C. in a medium comprising B-complex vitamin and amino-N with trace metals;
- and,
- instructions for use of the Geobacillus midousuji in the soil, sediment or wastewater, wherein the soil, sediment or wastewater is maintained at a temperature ranging from about 40° C. to about 70° C. and a humidity ranging from about 90% to about 100% for a period of time ranging from about 1 hour to about 20 days.
Dependencies are inferred from the claim text.