Multiplex pcr chip and multiplex pcr method using same

A multiplex PCR chip, comprising:

• a substrate made of a transparent plastic material;
• one or more reaction chambers famed on the substrate and configured to have a predetermined size and depth;
• and
• a plurality of particle-forming grooves formed in each reaction chamber and configured to have a size and depth capable of accommodating a predetermined amount of a probe in a liquid state,
• wherein the particle-forming grooves have various planar shapes and
• the probes injected into the particle-forming grooves having different planar shapes comprises:
• primers having different types and specifically hybridizing with sequences of different nucleic acid molecules;
• a fluorescent dye combined with the primers;
• and
• a supporting body to which the primers are fixed.

A multiplex PCR method, comprising:

• step (S110) of preparing a PCR chip, wherein the PCR chip provided with a plurality of reaction chambers connected to each other in series through a single connection channel and provided with a plurality of spatially separated particle-forming grooves formed inside each reaction chamber;
• step (S120) of injecting a probe, wherein the probe in a solution state is injected and provided with primers specifically hybridizing with sequences of different nucleic acid molecules in the plurality of particle-forming grooves, a fluorescent dye combined to the primers, and a supporting body forming a three-dimensional reticular structure when cured;
• step (S130) of fixing probe particles, wherein a physical stimulus is applied to the probe injected into each particle-forming groove to cure the supporting body, and at the same time, the primers are fixed to an inner side of the supporting body to form the probe particles having shapes respectively corresponding to the particle-forming grooves;
• and
• step (S140) of cleaning the probe particles, wherein pores are formed in the supporting body by injecting a cleaning solution into the plurality of reaction chambers to which the probe particles are respectively fixed.