Robotic method for coating a multiwell plate by a polyelectrolyte multilayer film
AssigneesInstitut Polytechnique De Grenoble, Centre National De La Recherche Scientifique
InventorsCatherine Cécile Picart, Jie Liu, Fabien Dalonneau, Paul Machillot
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Abstract
The invention concerns a robotic method for coating the bottom surface of at least one well of a multiwell plate by a polyelectrolyte multilayer film, the multiwell plate obtainable according to the method and the use thereof for cell culture.
Claims20
A multiwell plate obtainable by a method comprising n successive sequences, n being an integer from 1 to 2000, wherein each sequence comprises the steps of:
- a) robotic deposit of a volume VPE1 of a solution of a first polyelectrolyte PE1 on the bottom surface of at least one well of a multiwell plate, wherein the first polyelectrolyte PE1 is either a cationic polymer comprising amino groups, or an anionic polymer, then
- b) robotic aspiration of an aspirated volume VaspPE1 of said solution of PE1, wherein the aspirated volume VaspPE1 is higher than or equal to VPE1, then
- c) robotic deposit of a volume VPE2 of a solution of a second polyelectrolyte PE2 on said bottom surface, wherein the second polyelectrolyte PE2 is a cationic polymer comprising amino groups when PE1 is an anionic polymer, or PE2 is an anionic polymer when PE1 is a cationic polymer comprising amino groups, then
- d) robotic aspiration of an aspirated volume VaspPE2 of said solution of PE2, wherein the aspirated volume VaspPE2 is higher than or equal to VPE2.
A multiwell plate comprising wells, wherein the bottom surface of m wells is coated by a polyelectrolyte multilayer film,
- wherein m is an integer from 1 to the number of wells of the multiwell plate,
- the polyelectrolyte multilayer film comprising n layer pairs, n being an integer from 1 to 2000 and each layer pair comprising a layer of a first polyelectrolyte PE1 and a layer of a second polyelectrolyte PE2 of opposite charge,
- wherein the first polyelectrolyte PE1 is either a cationic polymer comprising amino groups, or an anionic polymer,
- the second polyelectrolyte PE2 is a cationic polymer comprising amino groups when PE1 is an anionic polymer, or PE2 is an anionic polymer when PE1 is a cationic polymer comprising amino groups,
- the polyelectrolyte multilayer film presenting a coefficient of variation CV of its mean thickness less than or equal to 20.3%,
- wherein
- CV
- =
- SD
- hMEAN
- ×
- 100
- SD being the standard deviation
- SD
- =
- ∑
- 1
- m
-
- (
- hWELLi
- -
- hMEAN
- )
- 2
- (
- m
- -
- 1
- )
- hMEAN
- =
- ∑
- 1
- m
-
- hWELL
- m
- h
- WELL
- =
- (
- hN
- +
- hW
- +
- hC
- +
- hE
- +
- hS
- )
- S
- hN, hW, hC, hE and hS being film thicknesses determined at the positions N, W, C, E, S inside each well as shown in FIG. 3.
A multiwell plate comprising wells, wherein the bottom surface of m wells is coated by a polyelectrolyte multilayer film,
- wherein m is an integer from 1 to the number of wells of the multiwell plate,
- the polyelectrolyte multilayer film comprising n layer pairs, n being an integer from 1 to 2000 and each layer pair comprising a layer of a first polyelectrolyte PE1 and a layer of a second polyelectrolyte PE2 of opposite charge,
- wherein the first polyelectrolyte PE1 is either a cationic polymer comprising amino groups, or an anionic polymer,
- the second polyelectrolyte PE2 is a cationic polymer comprising amino groups when PE1 is an anionic polymer, or PE2 is an anionic polymer when PE1 is a cationic polymer comprising amino groups,
- the polyelectrolyte multilayer film presenting a standard deviation SD less than or equal to 3.9%,
- wherein
- SD
- =
- ∑
- 1
- m
-
- (
- hWELLi
- -
- hMEAN
- )
- 2
- (
- m
- -
- 1
- )
- hMEAN
- =
- ∑
- 1
- m
-
- hWELL
- m
- h
- WELL
- =
- (
- hN
- +
- hW
- +
- hC
- +
- hE
- +
- hS
- )
- S
- hN, hW, hC, hE and hS being film thicknesses determined at the positions N, W, C, E, S inside each well as shown in FIG. 3.
Dependencies are inferred from the claim text.