Large Pore Agarose
AssigneeCytiva BioProcess R&D AB
InventorsDavid Jansson, Jesper Hansson, Anna Akerblom, Emma Michaelsson
Life of this patent
Estimating the term…
Abstract
The present invention relates to porous cross-linked agarose gel beads which have a low agarose content, a method for the preparation of the beads and their use in chromatographic applications. The beads are suitable for the separation/purification of biomolecules from a biological sample. Due to the high porosity of the beads, they are especially suitable for separation/isolation of larger particles, such as virus particles e.g. adeno virus.
Claims19
A method of preparing porous cross-linked agarose gel beads comprising the steps of:
- a) emulsifying agarose, comprising the steps of:
- (i) preparing an agarose aqueous phase having an agarose concentration of 0.3-0.8% w/v,
- (ib) adding magnetite,
- (ii) preparing a water-immiscible oil phase in which at least one emulsifier is dissolved,
- (iii) mixing the water phase and the oil phase to obtain a W/O emulsion, and
- (iv) allowing the W/O emulsion to form beads,
- b) cross-linking the emulsified agarose one or several times by reacting the beads with a cross-linking agent, and
- c) coupling of ligands.
Porous spherical cross-linked agarose gel beads, comprising an agarose concentration of about 0.3-0.8% w/v, or a dry weight of about 5-15 mg/mL, and a magnetic material.
Dependencies are inferred from the claim text.