Devices and methods for light-directed polymer synthesis
AssigneeTwist Bioscience Corporation
InventorsJeremy LACKEY, Scott INDERMUEHLE, David DODD, Stefan PITSCH
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Abstract
Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable LED arrays to control polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.
Claims28
A device for polymer synthesis, comprising:
- a solid support, wherein the solid support comprises a plurality of wells, wherein each of the wells comprises:
- a) a synthesis surface located in a bottom region of each of the wells;
- b) a light-emitting layer in addressable communication with the synthesis surface and situated below the synthesis surface;
- and
- c) a CMOS driver located in addressable communication with the light-emitting layer.
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A method for synthesizing a polymer, comprising:
- a) providing a solid support comprising a surface;
- b) depositing at least one nucleoside on the surface, wherein the at least one nucleoside couples to a polynucleotide attached to the surface, wherein the coupling comprises a light-directed deprotection step by a light-emitting layer, and wherein the light-emitting layer is located beneath the surface;
- and
- c) repeating step b) to synthesize a plurality of polynucleotides on the surface, wherein polynucleotides having different sequences on the surface are present at a density of at least 100×106 polynucleotides per cm2.
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Dependencies are inferred from the claim text.