Achromosomal dynamic active systems
AssigneeFlagship Pioneering Innovations VI, LLC
InventorsAdam Barclay FISHER, Kimberly A. HOMAN, Troy Patrick HUBBARD, David Barry KOLESKY, Analise Zaunbrecher REEVES, Caitlin Nicole SPAULDING, Hok Hei TAM
Life of this patent
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Abstract
The invention provides isolated achromosomal dynamic active systems (ADAS), including highly active ADAS. These ADAS provided by the invention can be obtained by a variety of means. Various associated methods of making and using these ADAS are provided.
Claims130
A method for manufacturing a composition comprising a plurality of ADAS, the composition being substantially free of viable bacterial cells, the method comprising:
- (a) making, providing, or obtaining a plurality of parent bacteria having a reduction in the level or activity of a cell division topological specificity factor;
- (b) exposing the parent bacteria to conditions allowing the formation of minicells;
- and
- (c) separating the minicells from the parent bacteria, thereby producing a composition comprising a plurality of ADAS that is substantially free of viable bacterial cells.
A composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1 mM and wherein the composition is substantially free of viable bacterial cells.
A composition comprising a plurality of highly active ADAS, wherein the ADAS have an initial ATP concentration of at least 3 mM and wherein the composition is substantially free of viable bacterial cells.
A composition comprising a plurality of ADAS, wherein the ADAS do not comprise a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells.
A composition comprising a plurality of ADAS, the composition being substantially free of viable bacterial cells, and being produced by a process comprising:
- (a) making, providing, or obtaining a plurality of parent bacteria having a reduction in the level or activity of a cell division topological specificity factor;
- (b) exposing the parent bacteria to conditions allowing the formation of minicells;
- and
- (c) separating the minicells from the parent bacteria, thereby producing a composition that is substantially free of viable bacterial cells.
The composition of claim 42, wherein the parent bacteria is Salmonella typhimurium and the minE polypeptide is S. typhimurium minE.
A method for delivering a highly active ADAS to a target cell, the method comprising:
- (a) providing a composition comprising a plurality of highly active ADAS, wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the target cell with the composition of step (a).
A method for delivering an ADAS to a target cell, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the target cell with the composition of step (a).
A method for delivering a cargo to a target cell, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM, the ADAS comprise a cargo, and the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the target cell with the composition of step (a).
A method for delivering a cargo to a target cell, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor, the ADAS comprise a cargo, and the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the target cell with the composition of step (a).
A method of modulating a state of an animal cell, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the animal cell with the composition of step (a), whereby a state of the animal cell is modulated.
A method of modulating a state of a plant cell, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the plant cell with the composition of step (a), whereby a state of the plant cell is modulated.
A method of modulating a state of an insect cell, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the insect cell with the composition of step (a), whereby a state of the insect cell is modulated.
A method of modulating a state of an animal cell, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the animal cell with the composition of step (a), whereby a state of the animal cell is modulated.
A method of modulating a state of a plant cell, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the plant cell with the composition of step (a), whereby a state of the plant cell is modulated.
A method of modulating a state of an insect cell, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the insect cell with the composition of step (a), whereby a state of the insect cell is modulated.
A method of treating an animal in need thereof, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the animal with an effective amount of the composition of step (a), thereby treating the animal.
A method of treating an animal in need thereof, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the animal with an effective amount of the composition of step (a), thereby treating the animal.
A method of treating a plant in need thereof, the method comprising:
- (a) providing a composition comprising a plurality of highly active achromosomal dynamic active systems (ADAS), wherein the ADAS have an initial ATP concentration of at least 1.25 mM and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the plant or a pest thereof with an effective amount of the composition of step (a), thereby treating the plant.
A method of treating a plant in need thereof, the method comprising:
- (a) providing a composition comprising a plurality of ADAS, wherein the ADAS are derived from parent bacteria having a reduction in the level or activity of a cell division topological specificity factor and wherein the composition is substantially free of viable bacterial cells;
- and
- (b) contacting the plant or a pest thereof with an effective amount of the composition of step (a), thereby treating the plant.
A composition comprising a plurality of ADAS, wherein the ADAS comprise an enzyme and wherein the enzyme alters a substrate to produce a target product.
Dependencies are inferred from the claim text.