Bacteriophage for modulating inflammatory bowel disease

8) A pharmaceutical composition comprising at least two different lytic bacteriophage, each selected from a different group, wherein the groups consist of:

• a) KP3W-P0101, KP3W-P0102, KP3W-P0103, KP3W-P0104, KP3W-P0105, KP3W-P0106, KP3W-P0107, KP3W-P0108, KP3W-P0109, KP3W-P0110, KP3W-P0111, KP3W-P0112, KP3W-P0113, KP3W-P0114, and any other lytic bacteriophage that has at least 78% homology as determined by BLAST to any of the foregoing bacteriophage in a) and is capable of lytic infection of KP3 or a KP3 mutant,
• b) KP3W-P0201, KP3W-P0202, KP3W-P0203, and any other lytic bacteriophage that has at least 96% homology as determined by BLAST to any of the foregoing bacteriophage in b) and is capable of lytic infection of KP3 or a KP3 mutant, and
• c) KP3W-S0101, KP3W-S0102, and any other lytic bacteriophage that has at least 88% homology as determined by BLAST to any of the foregoing bacteriophage in c) and is capable of lytic infection of KP3 or a KP3 mutant,
• wherein the pharmaceutical composition is formulated for delivery to a mammalian intestine or formulated for delivery to a mammalian mouth.

12) A pharmaceutical composition comprising at least two different lytic bacteriophage, wherein

• a) at least one bacteriophage is selected from:
• i) KP2W-P0101, KP2W-P0102, KP2W-P0103, KP2W-P0104, KP2W-P0105, KP2W-P0106, KP2W-P0107, KP2W-P0108, KP2W-P0109, any other lytic bacteriophage that has at least 83% homology to the foregoing bacteriophage of i) and is capable of lytic infection of KP2 or a KP2 mutant,
• ii) KP2M-P0201, KP2W-P0202, KP2M-P0203, KP2M-P0204, KP2M-P0205, KP2M-P0206, KP2M-P0207, KP2M-P0208, KP2M-P0209, KP2M-P0210, KP2M-P0211, KP2M-P0212, and any other lytic bacteriophage that has at least 85% homology as determined by BLAST to any of the foregoing bacteriophage of ii) and is capable of lytic infection of KP2 or a KP2 mutant, and
• iii) KP2W-M0102, KP2W-M0103, KP2W-M0104, KP2M-M0105, KP2W-M0106, KP2W-M0107, KP2M-M0108, KP2M-M0109, KP2M-M0110, KP2M-M0111, KP2M-M0112, any other lytic bacteriophage that has at least 86% homology as determined by BLAST to any of the foregoing bacteriophage of ii) and is capable of lytic infection of KP2 or a KP2 mutant, and
• b) at least one bacteriophage is selected from:
• i) KP3W-P0101, KP3W-P0102, KP3W-P0103, KP3W-P0104, KP3W-P0105, KP3W-P0106, KP3W-P0107, KP3W-P0108, KP3W-P0109, KP3W-P0110, KP3W-P0111, KP3W-P0112, KP3W-P0113, KP3W-P0114, and any other lytic bacteriophage that has at least 78% homology as determined by BLAST to any of the foregoing bacteriophage of i) and is capable of lytic infection of KP3 or a KP3 mutant,
• ii) KP3W-P0201, KP3W-P0202, KP3W-P0203, and any other lytic bacteriophage that has at least 96% homology as determined by BLAST to any of the foregoing bacteriophage of ii) and is capable of lytic infection of KP3 or a KP3 mutant,
• iii) KP3W-S0101, KP3W-S0102, and any other lytic bacteriophage that has at least 88% homology as determined by BLAST to any of the foregoing bacteriophage of iii) and is capable of lytic infection of KP3 or a KP3 mutant,
• wherein the pharmaceutical composition is formulated for delivery to a mammalian intestine or formulated for delivery to a mammalian mouth.

27) A method of determining a subject to be treated with a KP2 bacteriophage, comprising the steps of:

• a) obtaining a biological sample from the subject;
• b) culturing bacteria obtained from the biological sample;
• c) inoculating the cultured bacteria with a bacteriophage selected from one or more of:
• i) KP2W-P0101, KP2W-P0102, KP2W-P0103, KP2W-P0104, KP2W-P0105, KP2W-P0106, KP2W-P0107, KP2W-P0108, KP2W-P0109, and any other lytic bacteriophage that has at least 83% homology as determined by BLAST to any of the foregoing bacteriophage of i) and is capable of lytic infection of KP2 or a KP2 mutant,
• ii) KP2M-P0201, KP2W-P0202, KP2M-P0203, KP2M-P0204, KP2M-P0205, KP2M-P0206, KP2M-P0207, KP2M-P0208, KP2M-P0209, KP2M-P0210, KP2M-P0211, KP2M-P0212, and any other lytic bacteriophage that has at least 85% homology as determined by BLAST to any of the foregoing bacteriophage of ii) and is capable of lytic infection of KP2 or a KP2 mutant, and
• iii) KP2W-M0102, KP2W-M0103, KP2W-M0104, KP2M-M0105, KP2W-M0106, KP2W-M0107, KP2M-M0108, KP2M-M0109, KP2M-M0110, KP2M-M0111, KP2M-M0112, and any other lytic bacteriophage that has at least 86% homology as determined by BLAST to any of the foregoing bacteriophage of iii) and is capable of lytic infection of KP2 or a KP2 mutant, and
• d) determining whether the cultured bacteria are lysed by the bacteriophage,
• wherein when any of the cultured bacteria are lysed by the bacteriophage, the subject is determined to be infected by KP2.

29) A method of determining a subject to be treated with a KP3 bacteriophage, comprising the steps of:

• a) obtaining a biological sample from the subject;
• b) culturing bacteria obtained from the biological sample;
• c) inoculating the cultured bacteria with a bacteriophage selected from one or more of:
• i) KP3W-P0101, KP3W-P0102, KP3W-P0103, KP3W-P0104, KP3W-P0105, KP3W-P0106, KP3W-P0107, KP3W-P0108, KP3W-P0109, KP3W-P0110, KP3W-P0111, KP3W-P0112, KP3W-P0113, KP3W-P0114, and any other lytic bacteriophage that has at least 78% homology as determined by BLAST to any of the foregoing bacteriophage of i) and is capable of lytic infection of KP3 or a KP3 mutant,
• ii) KP3W-P0201, KP3W-P0202, KP3W-P0203, and any other lytic bacteriophage that has at least 96% homology as determined by BLAST to any of the foregoing bacteriophage of ii) and is capable of lytic infection of KP3 or a KP3 mutant, and
• iii) KP3W-S0101, KP3W-S0102, and any other lytic bacteriophage that has at least 88% homology as determined by BLAST to any of the foregoing bacteriophage of iii) and is capable of lytic infection of KP3 or a KP3 mutant, d) determining whether the cultured bacteria are lysed by the bacteriophage, wherein when any of the cultured bacteria are lysed by the bacteriophage, the subject is determined to be infected by KP3.