Compositions and methods for reducing inflammation
AssigneeHistogen, Inc.
InventorsGail K. Naughton, Martin Latterich, Michael Zimber
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Abstract
The present invention is directed to a method of producing compositions derived from culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both an extracellular matrix composition and a conditioned culture medium composition, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
Claims21
A method for reducing inflammation in a subject comprising administering to the subject a conditioned culture medium (CCM) composition or an extracellular matrix (ECM) composition, wherein the CCM and ECM compositions are produced by the method comprising culturing fibroblast cells on microcarrier beads or a three-dimensional surface under hypoxic conditions of 1-5% oxygen, thereby producing multipotent stem cells which produce a CCM and an ECM fraction, thereby reducing inflammation.
A method of treating intervertebrate disc degeneration (IDD) in a subject comprising administering to the subject a cell conditioned media (CCM) composition and/or an extracellular matrix (ECM) composition, wherein the CCM and ECM compositions are produced by the method comprising culturing fibroblast cells on microcarrier beads or a three-dimensional surface under hypoxic conditions of 1-5% oxygen, thereby producing multipotent stem cells which produce a CCM and an ECM fraction, thereby treating IDD.
An anti-inflammatory composition comprising a CCM composition or an ECM composition produced by the method comprising culturing fibroblast cells on microcarrier beads or a three-dimensional surface under hypoxic conditions of 1-5% oxygen, thereby producing multipotent stem cells which produce a soluble and a non-soluble fraction and a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.