Improved delivery of large agents

A patch comprising a composition comprising a large agent and a plurality of microneedles having a microneedle density within a range of about 2 to about 50 microneedles/cm2.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array having a microneedle hole puncture size within a range of about 100 to about 35,000 μm2/microneedle.

A kit comprising a composition comprising a large agent and a device for microneedle conditioning of a site, wherein the device is a microneedle array having a microneedle density within a range of about 2 to about 50 microneedles/cm2.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site that has been conditioned with microneedle skin conditioning (MSC) of the site with a microneedle array having a microneedle density within a range of about 2 to about 50 microneedles/cm2.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site that has been conditioned with microneedle skin conditioning (MSC) of the site with a microneedle array having a microneedle hole puncture size within a range of about 100 to about 35,000 μm2/microneedle.

A kit comprising a composition comprising a large agent and a device for microneedle conditioning of a site, wherein the device is a microneedle array having a microneedle hole puncture size within a range of about 100 to about 35,000 μm2/microneedle.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array wherein between about 1 to about 13 impressions of the microneedle array are made to the site.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array wherein between about 1 to about 4 impressions of the microneedle array are made per square centimeter of the site.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array having a microneedle length within a range of about 1 μm to about 700 μm.

A method comprising a step of:

• applying an emulsion composition comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array having a microneedle length within a range of about 1 μm to about 500 μm.

A method comprising a step of:

• applying an emulsion composition of a product volume of about 1/100 of one drop/cm2 to about 2 drops/cm2 comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array.

A method comprising a step of:

• applying an emulsion composition of a product volume of about 0.0001 mls/cm2 to about 0.065 mls/cm2 comprising a large agent having a molecular weight of 100,000 Da or greater to a site in combination with microneedle skin conditioning (MSC) of the site with a microneedle array.