Method and device for measuring the status of oxidative stress in a biological matrix
OwnerSmail Mezianeinventor
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Abstract
Methods and devices for measuring the status of oxidative stress on the surface or in a biological matrix involve use of at least one compound selected from NADH, NADPH, Cyt C (Fe2+) or H2O2.
Claims13
A method for measuring a status of an oxidative stress of a biological matrix, comprising:
- using at least one compound that is non-toxic to the skin chosen from NADH, NADPH, Cyt C (Fe2+) or H2O2 for measuring the status of the oxidative stress of the biological matrix, wherein the at least one compound is available in two forms A that do not interact with each other, and the two forms are capable of interacting and/or forming complexes respectively with an antioxidant and an oxidant present on the surface of the biological matrix or in the biological matrix.
A device for measuring the total antioxidant/oxidizing power of a biological matrix comprising a measuring medium in which are immersed one or more working electrodes and one or more reference electrodes, wherein the measuring medium comprises at least one compound available in two forms, which are capable of interacting and/or of forming complexes respectively with antioxidants and oxidants present at the surface or in the matrix, and not interacting with each other, and wherein the at least one compound is chosen from NADH, NADPH, Cyt C (Fe2+) or H2O2.
A method for measuring the status of oxidative stress of a biological matrix, comprising the steps of:
- bringing the biological matrix into contact with a measuring medium comprising at least one compound chosen from NADH, NADPH, Cyt C (Fe2+) or H2O2, available in two forms that are capable of interacting and/or forming complexes respectively with an antioxidant and an oxidant present at the surface of the biological matrix or in the biological matrix;
- and
- measuring the electrochemical potential between a complex working electrode or several working electrodes and one or more reference electrodes placed on the biological matrix by electrochemistry, or measuring a variation in the absorbance by spectrophotometry.
Dependencies are inferred from the claim text.