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US 2022/0030788 A1Published application
Genome edited fine mapping and causal gene identification
AssigneePioneer Hi-Bred International, Inc.
InventorsSABRINA HUMBERT, MARK TIMOTHY JUNG, ZHAN-BIN LIU, ROBERT B MEELEY, BO SHEN, MARISSA SIMON, PETRA J WOLTERS
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Abstract
The field is molecular biology, and more specifically, methods for editing the genome of a plant cell to identify causal alleles of a desired trait or to fine map a desired trait to small region of the genome for gene identification.
Claims41
A method for fine mapping a desired trait comprising:
- a) introducing a site-specific modification in at least one target site in an endogenous genomic locus in a plant;
- b) obtaining the plant having a modified nucleotide sequence;
- and
- c) screening for the site-specific modification;
- and
- d) screening for an increase or decrease in a phenotype of the desired trait.
3 more elements
A method for identifying a causal gene of a desired trait comprising:
- a) introducing at least one site-specific modification in an endogenous genomic locus in a plant;
- b) obtaining the plant having at least one site-specific modification;
- c) screening the plant or the plant's progeny for the presence or absence of the desired trait;
- and
- d) identifying the causal gene.
2 more elements
A method to create a novel haplotype in a genomic locus comprising:
- a) introducing at least one site-specific modification in an endogenous genomic locus in a first plant;
- b) screening for the site-specific modification;
- and
- c) correlating the haplotype with a phenotype to establish a cause and effect relationship between the at least one site-specific modification and the desired trait.
1 more element
-79. (canceled)
Dependencies are inferred from the claim text.