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US 2022/0026696 A1Published application
Light sheet microscope with turreted lenses
AssigneeLaVision BioTec GmbH
InventorsHeinrich Spiecker, Andreas HEYMANN
Life of this patent
Estimating the term…
Abstract
The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.
Claims22
A light sheet microscope for imaging a first biological sample disposed on a sample holder, comprising:
- an imaging lens structure including an operative objective lens having a focal plane and at least one inoperative lens, and wherein the operative lens is movable in a z-direction orthogonal to the focal plane by a movable first stage;
- a container holding a quantity of fluid, wherein the sample holder is immersible in the fluid by moving the container in the z-direction, orthogonal to the focal plane;
- and
- a detector which forms an image of the focal plane, wherein the image includes at least a portion of the first biological sample, and wherein the movable first stage has sufficient range of motion to submerge the operative objective lens into the fluid held in the container, thereby forming an image of the first biological sample on the detector, and wherein the sample holder and container have a shape which admits a movement of the imaging lens structure, when the operative lens is submerged.
1 more element
(canceled)
A method for using a light sheet microscope having a rotating turret on with a plurality of imaging lenses is disposed thereon, comprising:
- forming an image on a detectors by adjusting a position of an operative objective lens, with the operative objective lens having its optical axis in a z-direction perpendicular to the focal plane, and wherein the focal plane includes at least a portion of a sample holder, wherein the sample holder holds a biological sample in a clearing fluid and the operative objective lens is submerged in the clearing fluid;
- moving the operative objective lens in the z-direction to withdraw the operative objective lens from the clearing fluid;
- rotating the rotating turret to move another of the imaging lenses into the imaging position without changing the focal plane or the position of the sample holder.
22-24. (canceled)
Dependencies are inferred from the claim text.