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US 2022/0026428 A1Published application
Detection of antibodies to sarsr-cov
OwnerLinfa WANGinventor
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Abstract
A kit, composition and method for detection of antibodies to severe acute respiratory syndrome related coronavirus (SARSr-CoV), and for diagnosis of SARSr-CoV infection.
Claims26
A method of analysing a sample for the presence of antibodies to a SARSr-CoV, comprising:
- contacting the sample with:
- (i) a spike protein of a severe acute respiratory syndrome-related coronavirus (SARSr-CoV), a S1 subunit or a receptor binding domain (RBD) of a SARSr-CoV spike protein, wherein the spike protein or the S1 subunit comprises the RBD of the SARSr-CoV spike protein, and (ii) an ACE2 protein or an extracellular domain of an ACE2 protein or a fragment thereof which binds specifically to the spike protein, the S1 subunit or the RBD of (i), and
- determining the level of interaction between the spike protein, the S1 subunit or the RBD of (i) and the ACE2 protein or the ACE2 protein extracellular domain of (ii),
- wherein the spike protein, the S1 subunit or the RBD of (i) or ACE2 protein or the ACE2 protein extracellular domain of (ii) is conjugated to a) a detection entity, or b) a biotin which binds an avidin or streptavidin-conjugated detection entity, for determining the interaction between (i) and (ii);
- and
- (a) the spike protein, the S1 subunit or the RBD of (i) is conjugated to the detection entity or the biotin, and wherein the ACE2 protein or the ACE2 protein extracellular domain of (ii) is immobilised on a solid support;
- or
- (b) the ACE2 protein or the ACE2 protein extracellular domain of (ii) is conjugated to the detection entity or the biotin, and wherein spike protein, the S1 subunit or the RBD of (i) is immobilised on a solid support.
5 more elements
A method of determining whether a subject is or has been infected with a SARSr-CoV, comprising:
- contacting a sample obtained from the subject with:
- (i) a spike protein of a SARSr-CoV, a S1 subunit or a RBD of SARSr-CoV spike protein, wherein the spike protein or the S1 subunit comprises the RBD of the SARSr-CoV spike protein, and (ii) an ACE2 protein or an extracellular domain of an ACE2 protein or a fragment thereof which binds specifically to the spike protein, the S1 subunit or the RBD of (i), and
- determining the level of interaction between the spike protein, the S1 subunit or the RBD of (i) and the ACE2 protein or the ACE2 protein extracellular domain of (ii),
- wherein the spike protein, the S1 subunit or the RBD of (i) or ACE2 protein or the ACE2 protein extracellular domain of (ii) is conjugated to a) a detection entity or b) a biotin which binds an avidin or streptavidin-conjugated detection entity, for determining the interaction between (i) and (ii);
- and
- (a) the spike protein, the S1 subunit or the RBD of (i) is conjugated to the detection entity or the biotin, and wherein the ACE2 protein or the ACE2 protein extracellular domain of (ii) is immobilised on a solid support;
- or
- (b) the ACE2 protein or the ACE2 protein extracellular domain of (ii) is conjugated to the detection entity or the biotin, and wherein spike protein, the S1 subunit or the RBD of (i) is immobilised on a solid support.
5 more elements
Dependencies are inferred from the claim text.