A process for production of nigericin from streptomyces sp. mcc-0151
AssigneeCouncil of Scientific and Industrial Research
InventorsGulam Dastager Syed, Amit Kumar Sahu
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Abstract
A fermentation process is provided for the synthesis of Nigericin from Streptomyces sp. having accession number MCC 0151 and its isolation with high yield. A microbial inoculant composition is provided, comprising a biologically pure culture of Streptomyces sp. MCC 0151 for the exclusive production of Nigericin with high yield.
Claims9
A process for synthesis of nigericin from Streptomyces sp. MCC 0151, the process comprising:
- (i) culturing an inoculum comprising Streptomyces sp. MCC 0151 in a metabolite production medium;
- (ii) mixing the metabolite-production medium containing the Streptomyces sp. MCC 0151 and ethyl acetate in a ratio of 1:1 to obtain a mixture;
- (iii) centrifuging the mixture obtained in (ii) to obtain a supernant and an ethyl acetate extract comprising nigericin;
- (iv) evaporating the ethyl acetate extract obtained in (iii) to obtain a dried crude extract;
- and
- (v) purifying the dried crude extract obtained in (iv) by column chromatography to obtain a purified nigericin, wherein nigericin is produced having a high yield with concentration up to 500 mg/L.
A microbial composition comprising a biologically pure culture of Streptomyces sp. having accession number MCC-0151 in a concentration from 5% to 10% by weight of the metabolite production medium and a suitable excipient.
Dependencies are inferred from the claim text.