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US 2022/0025404 A1Published application
Compositions and methods for monitoring in real-time construction and bioengineering of mammalian synthetic chromosomes
AssigneeCarryGenes Bioengineering, LLC
InventorsEdward PERKINS, Amy GREENE
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Abstract
The present invention encompasses compositions and methods to allow one to monitor formation of synthetic chromosomes in real-time via standardized fluorescent technology, eliminating the need for cumbersome, expensive, and possibly mutagenic analysis.
Claims20
A mammalian synthetic chromosome production host cell line for tracking synthetic chromosome production in real-time, comprising:
- (i) an endogenous chromosome tag that labels one or more endogenous chromosomes in the chromosome production host cell line;
- and
- (ii) a differently-labeled synthetic chromosome tag that identifies, apart from the endogenous chromosomes, a synthetic chromosome as it is being produced,
- wherein the endogenous chromosome tag and synthetic chromosome tag are stably integrated into the synthetic chromosome production host cell line's genome.
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Dependencies are inferred from the claim text.