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US 2022/0025387 A1Published application
Multi-Copy Gene Protein Expression System
AssigneeNovartis AG
InventorsClemens Achmüller, Norbert Lamping, Matjaz Vogelsang
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AssigneeNovartis AG
InventorsClemens Achmüller, Norbert Lamping, Matjaz Vogelsang
Estimating the term…
The present invention belongs to the field of biotechnology, specifically to the field of recombinant protein expression. The present invention focuses on two problems commonly encountered during recombinant protein expression, low quantity of protein expression and genetic instability of cell lines used for recombinant protein expression. The basic principle of the present invention is to introduce several expression cassettes into a cell which expression cassettes all code for the same mature recombinant protein of interest, but which expression cassettes have different nucleotide sequences. Expression cassette means a polynucleotide sequence which comprises at least a promoter sequence, a start codon, a polynucleotide sequence coding for a protein which is intended to be recombinant expressed (POI), a stop codon and a terminator.
Host cell comprising three or more different types of expression cassettes, each expression cassette coding for the same Protein Of Interest (POI) with identical mature amino acid sequence, and each type of expression cassette at least is comprising a promoter sequence, a polynucleotide sequence of the coding sequence of the POI, and a terminator sequence, wherein said expression cassettes differ in that they comprise
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