US 2022/0025371 A1 · CMI SEQUENCES AS AN EARLY MARKER FOR THE FUTURE DEVELOPMENT OF CANCER, ATHEROSCLEROSIS, DIABETES AND DISEASES OF THE CNS AND AS A TARGET FOR THE TREATMENT AND PREVENTION OF THESE DISEASES

Abstract

Described are CMI (Cow Milk Isolate) nucleotide sequences as well as probes and primers comprising part of said nucleotide sequences and antibodies against polypeptides encoded by said nucleotide sequences. Said compounds are useful as early markers for the future development of cancer, diabetes, atherosclerosis and diseases of the CNS (Multiple sclerosis MS, Prion-linked diseases, amyotrophic lateral sclerosis, transmissible spongiforme encephalitis, Parkinson's disease, Alzheimer disease) and should represent targets for treatment and prevention.

Claims18

Dependent claims

A method for isolating DNA from a cow milk sample, comprising the steps of

(a) phenol-chloroform extraction of DNA from the cow milk sample followed by ethanol precipitation,
(b) performing rolling circle amplification (RCA) with the DNA extracted in step (a),
(c) inverted PCR using 2 abutting primer pairs A and B selected from
Primer pair A:
>LSconAfwd:
aaggcagatcaacacagg
>LSconArev:
Agcagattgcaaagcctg
Primer Pair B:
>LSconBfwd:
caacacagggatagaataac
>LSconBrev:
atctgccttagcagattgc

10 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/371,357Filed 9 Jul 2021

Priority

EP 19153535.024 Jan 2019

Published

27 Jan 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 15/113
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: non-coding nucleic acids modulating the…
C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
A61K 39/0011
Vertebrate antigens: cancer antigens
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…

Cmi sequences as an early marker for the future development of cancer, atherosclerosis, diabetes and diseases of the cns and as a target for the treatment and prevention of these diseases

Life of this patent

Cmi sequences as an early marker for the future development of cancer, atherosclerosis, diabetes and diseases of the cns and as a target for the treatment and prevention of these diseases

Life of this patent

Abstract

Claims18