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US 2022/0025353 A1Published application
Nanopore membrane device and methods of use thereof
OwnerJennifer A. Doudna, Yuhong Cao +2inventors
InventorsJennifer A. Doudna, Yuhong Cao, Enbo Ma, Peidong Yang
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Abstract
The present disclosure provides devices and methods for delivering a biomolecule into a cell. A delivery device of the present disclosure includes a first reservoir, a second reservoir, a porous membrane comprising a nanopore, and two or more electrodes configured to generate an electric field across the porous membrane for delivery of a biomolecule present in the second reservoir through the nanopore of the porous membrane and into a cell present in the first reservoir.
Claims22
A delivery device for delivering a biomolecule into a eukaryotic cell, the device comprising:
- a first reservoir comprising a proximal end and a distal end;
- a second reservoir comprising a proximal end and a distal end;
- a porous membrane comprising at least one nanopore with a pore size ranging from about 50 nm to about 150 nm, wherein the at least one nanopore is fluidically connected to the first reservoir and the second reservoir;
- and
- two or more electrodes configured to generate an electric field across the porous membrane.
2 more elements
Dependencies are inferred from the claim text.