In vitro method of differentiating a human pluripotent stem cell population into a cardiomyocyte cell population
AssigneeNcardia B.V.
InventorsStefan Robbert Braam, Ana Catarina Martins Grandela
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Abstract
The current invention relates to a method of differentiation of human pluripotent stem cells into a human stem-cell derived population of cardiomyocytes. The method comprises the use of specific combination of steps and compounds to induce and/or promote differentiation. The method also comprises steps directed to further maturation of the cardiomyocytes obtained with the method of the invention. Also provided are kits for use in a method of differentiation as well as cell populations obtainable with the method disclosed.
Claims25
An in vitro cardiomyocyte cell population produced by contacting a pluripotent stem cell (PSC) population in an aqueous media with a first compound that stimulates beta-catenin degradation and a second compound that suppresses phosphorylation of dishevelled 2.
A cell culture media comprising a first compound that stimulates beta-catenin degradation and a second compound that suppresses phosphorylation of dishevelled 2.
An in vitro human cardiomyocyte cell population in defined serum-free media wherein at least 80%, 90% or 95% of the cells express myosin light chain 2v, and cardiac troponin T.
A kit for differentiating a human pluripotent stem cell population into a cardiomyocyte cell population the kit comprising a) XAV939;
- IWP-L6;
- and an aqueous media;
- or b) a first aqueous media comprising CHIR-99021;
- a second aqueous media comprising XAV-939 and IWP-L6;
- and a third aqueous media that does not comprise XAV939, IWP-L6, and a Wnt-signaling agonist.
Dependencies are inferred from the claim text.