Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins
AssigneeCUBE BIOTECH GmbH
InventorRoland Fabis
Life of this patent
Estimating the term…
Abstract
A solid-phase chelator material usable for the purification of proteins. The solid-phase chelator material comprises a solid phase, polyamine groups bound to the solid phase and chelating groups bound to the polyamine groups. At least a part of the polyamine groups is connected with at least two chelating groups per polyamine group. Each chelating group comprises one or several aminopolycarboxylic acid groups (APA groups), with the proviso that the number of APA groups per polyamine group connected with at least two cheating groups is at least three.
Claims23
A solid-phase chelator material comprising a solid phase, polyamine groups bound to the solid phase and chelating groups bound to the polyamine groups, wherein at least a part of the polyamine groups are connected with at least two chelating groups per polyamine group and wherein each chelating group comprises one or several aminopolycarboxylic acid groups (APA groups), with the proviso that the number of APA groups per polyamine group connected with at least two chelating groups is at least three.
The solid-phase chelator material according to one of the preceding claims, wherein
- the bifunctional linker moiety K is selected from the group consisting of A-(CH2)n—B, A-Q-B and A-(C2H4O)m—(CH2)o—B, in which A and B are functional groups which are able react with carboxy groups, namely OH, NH2, NHR, Cl, Br, I, OMs, OTs, N3, wherein R is a hydrocarbon group with the formula CnH2n+1, in which n is an integer in the range from 1 to 6, m is an integer in the range from 1 to 12, o is an integer in the range from 2 to 12, Q is a straight or branched configuration of 2 to 100 atoms, optionally containing C, H, N, O and S, including cyclic compounds, such as carbohydrates, preferably a diamine NH2 or NHR which is more preferably selected from the group consisting of ethylenediamine, 1,4-diaminobutane, 1,6-diaminohexane, 1,8-diaminooctane, and Jeffamine ED-600;
- and
- the trifunctional linker moiety L has one of the general formulas
- in which A, D and E are functional groups which are able to react with a carboxy group, preferably —NH2 or —NHR, B is a branching atom, preferably C or N, which is connected with at least three stable bonds preferably to carbon atoms, connected with functional groups, n, m and o are integers in the range from 2 to 30;
- and is preferably a triamine, which is more preferably selected from the group consisting of diethylene triamine, tris(2-aminoethyl)amine, tris(2-aminopropyl)amine and amino-functionalized trifunctional branched PEGs based on a glycerol core.
Dependencies are inferred from the claim text.