RGMa Fragment Based Diagnostic Assay

A method of treating a subject suffering from neurodegenerative disease, the method comprising:

• (a) determining the levels of at least one RGMa fragment in a sample from the subject using the method comprising
• (a1) obtaining a sample from a subject comprising at least one RGMa fragment
• (a2) contacting the sample with a capture binding protein, wherein the capture binding protein binds to the at least one RGMa fragment to form a capture binding protein-RGMa fragment complex;
• (a3) contacting the sample with a detection binding protein, wherein the detection binding protein interacts with the capture binding protein to form a detection binding protein-capture binding protein RGMa fragment complex, and
• (a4) detecting and quantifying the at least one RGMa fragment in the sample,
• (b) comparing the levels of the at least one RGMa fragment in a sample from the subject to a control level of the at least one RGMa fragment;
• and
• (c) administering a treatment regimen to the subject if the levels of the fragments are increased compared to control levels.