Serine hydrolase profiling assay in biotherapeutics
OwnerGregory C. Adam, Xuanwen Li +2inventors
InventorsGregory C. Adam, Xuanwen Li, Simon Letarte, Douglas D. Richardson, II
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Abstract
The present disclosure describes a method of identifying serine hydrolase in a biological test sample obtained from protein production with a fluorophosphonate-containing probe. The present disclosure also provides a method of identifying one or more serine hydrolases in the biological test sample as causing PS-80 or PS-20 degradation.
Claims12
A method of identifying serine hydrolase in a biological test sample obtained from recombinant protein production, comprising the steps of:
- a. adding a fluorophosphonate probe to the biological test sample comprising a serine hydrolase in conditions that allow the probe to bind to the serine hydrolase;
- b. removing the free fluorophosphonate probes not bound to the serine hydrolase;
- c. enriching the probe-bound serine hydrolases with an agent that binds to the probes;
- d. digesting the serine hydrolase;
- and
- e. identifying the serine hydrolase in the test sample with mass spectroscopy.
A method of identifying one or more serine hydrolases in a biological test sample causing PS-80 or PS-20 degradation, wherein the biological test sample is obtained from recombinant protein production, comprising the steps of:
- a. adding a fluorophosphonate probe to the biological test sample that exhibits PS-80 or PS-20 degradation in conditions that allow the probe to bind to the serine hydrolase;
- b. removing the free fluorophosphonate probes not bound to the serine hydrolase;
- c. enriching the probe-bound serine hydrolases with an agent that binds to the probes;
- d. digesting the serine hydrolase;
- and
- e. identifying the one or more serine hydrolases in the test sample with mass spectroscopy.
Dependencies are inferred from the claim text.