Methods of screening
AssigneePhoremost Limited
InventorsBryn Shaun HARDWICK, Grahame James MCKENZIE
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Abstract
The invention provides a method of identifying a peptide interaction site on a target protein wherein the target protein modulates the phenotype of a mammalian cell, using mammalian encoded peptides (SEPs) such as short open reading frame (sORF) encoded peptides. The invention further provides a method for the identification of new therapeutic targets and protein interaction sites for use in drug discovery.
Claims20
1-16. (canceled)
A method of preparing library of nucleic acid molecules for intracellular expression of short peptides having lengths of fewer than 150 amino acids in mammalian cells, the method comprising:
- designing, in silico, a diverse population of bespoke short peptides having lengths of fewer than 150 amino acids;
- and
- constructing the library from nucleic acid molecules that encode the short peptides by cloning the nucleic acid molecules into an expression vector adapted for intracellular expression of the short peptides in mammalian cells.
Dependencies are inferred from the claim text.