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US 2022/0017973 A1Published application
Compositions and methods for detection of candida auris
AssigneeRoche Molecular Systems, Inc.
InventorsEllen H. FISS HOBART, Jody Harris, Andrew T. Hill, Jingtao Sun
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Abstract
Methods for the rapid detection of the presence or absence of Candida auris (CA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the CA 5.8s/ITS2 rRNA gene, along with kits are provided that are designed for the detection of CA.
Claims15
A method of detecting Candida auris (CA) in a sample, the method comprising:
- performing an amplifying step comprising contacting the sample with a set of CA 5.8s/ITS2 rRNA gene primers to produce an amplification product if CA 5.8s/ITS2 rRNA nucleic acid is present in the sample;
- performing a hybridizing step comprising contacting the amplification product with one or more detectable CA 5.8s/ITS2 rRNA gene probes;
- and
- detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of CA in the sample and wherein the absence of the amplification product is indicative of the absence of CA in the sample;
- wherein the set of CA 5.8s/ITS2 rRNA gene primers comprises a first oligonucleotide primer comprising a first nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-3, or a complement thereof, and a second oligonucleotide primer comprising a second nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 5-6, and 8 or a complement thereof;
- and
- wherein the one or more detectable CA 5.8s/ITS2 rRNA gene oligonucleotide probe comprises a third nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 9-11, or the complement thereof.
7 more elements
A kit for detecting a nucleic acid of Candida auris (CA) comprising:
- a first oligonucleotide primer comprising a first nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 1-3, or a complement thereof;
- a second oligonucleotide primer comprising a second nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 5-6, and 8 or a complement thereof;
- and
- a fluorescently detectably labeled oligonucleotide probe comprising a third nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 9-11, or a complement thereof, the detectably labeled probe configured to hybridize to an amplicon generated by the first and the second oligonucleotide primer.
4 more elements
Dependencies are inferred from the claim text.