Electroanalytical determination of leukocyte esterase
AssigneeBoard of Regents, The University of Texas System
InventorsWaldemar Gorski, Michael Bekhit
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Abstract
Disclosed are compositions and methods for detecting leukocyte esterase (LE) activity in a sample. The method can include contacting the sample with an assay sample comprising methyl pyruvate and alcohol oxidase to form a test sample, measuring H2O2 produced from contacting the sample and the assay sample in the test sample.
Claims22
A method for detecting leukocyte esterase (LE) activity in a sample, the method comprising:
- (a) contacting the sample with an assay sample comprising methyl pyruvate and alcohol oxidase to form a test sample;
- and
- (b) measuring concentration of H2O2 produced from contacting the sample and the assay sample in the test sample.
An leukocyte detection system comprising:
- an assay chamber configured to (i) contain an assay solution comprising methyl pyruvate and alcohol oxidase and (ii) be electronically coupled to an electrode and an electronic detector.
-26. (canceled)
A kit comprising an assay solution comprising methyl pyruvate and alcohol oxidase and a dipstick comprising affinity agents that bind and localize leukocyte esterase.
-30. (canceled)
Dependencies are inferred from the claim text.