Guide rna for hsv-1 gene editing and method thereof
AssigneeOriengene Biotechnology Ltd
InventorsXiaoming Li, Xiao Shu, Ru Jia, Ning Wang
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Abstract
The invention relates to a guide RNA (gRNA) comprising a guide sequence capable of targeting a Cas9 protein to a target sequence in an ICP6 gene of type 1-herpes simplex virus (HSV-1) to provide a specific cleavage event, wherein the target sequence comprises a GATC insertion as compared to a wild-type HSV-1. The invention also relates to a HSV-1 gene-editing system and a gene-editing method for generating a desired recombinant HSV-1 by using said gRNA.
Claims30
A guide RNA (gRNA) comprising a guide sequence capable of targeting a Cas9 protein to a target sequence in an ICP6 gene of type 1 herpes simplex virus (HSV-1) to provide a cleavage event, wherein the target sequence comprises a GATC insertion as compared to a wild-type HSV-1.
Dependencies are inferred from the claim text.