Method, Kit, and Device for Preparing Glycan from Glycoprotein

A kit for preparing a glycan from a glycoprotein, comprising:

• (a) a hydroxylamine compound;
• (b) a basic reagent;
• and
• (c) a purifying agent for preparing a glycan having a length of a monosaccharide or more, the purifying agent comprising a compound having a betaine structure.

A device for preparing a glycan from a glycoprotein, comprising:

• a first container holding portion configured to hold a first container in which a glycoprotein-containing sample that contains the glycoprotein is accommodated;
• a reagent introducing portion configured to introduce a reagent into the first container;
• and
• a second container holding portion configured to hold a second container that contains a purifying agent for purifying a glycan having a length of a monosaccharide or more, the purifying agent comprising a compound having a betaine structure;
• wherein the reagent introducing portion includes a glycan releasing reagent introducing portion configured to introduce a glycan releasing reagent that contains a hydroxylamine compound and a basic reagent into the first container.