High-affinity tcr for afp recognition

A T cell receptor (TCR), which has an activity of binding to FMNKFIYEI-HLA A0201 complex, and the T cell receptor comprises a TCR α chain variable domain and a TCR β chain variable domain, the TCR α chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR α chain variable domain are listed as follows,

• CDR1α:
• TSINN
• CDR2α:
• IRSNERE
• CDR3α:
• ATDEANDYKLS, and the TCR β chain variable domain comprises three CDR regions, and the reference sequences of the three CDR regions of the TCR β chain variable domain are listed as follows,
• CDR1β:
• SGDLS
• CDR2β:
• YYNGEE
• CDR3β:
• ASRGIGDAGELF
• the TCR α chain variable domain contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• A at position 5 of CDR3α
• V or Q or Y or H or T or W or R or D or
• L or E
• N at position 6 of CDR3α
• Y or L or H or I or Q or F or M or W or
• T or A
• D at position 7 of CDR3α
• T or Q or E or R or A or H or M or W or
• K or S or G
• Y at position 8 of CDR3α
• P or A
• K at position 9 of CDR3α
• A or G
• S at position 11 of CDR3α
• Y
• and/or, the TCR β chain variable domain contains at least one of the following mutations:
• Residue before mutation
• Residue after mutation
• S at position 1 of CDR1β
• P or K
• L at position 4 of CDR1β
• I
• S at position 5 of CDR1β
• P or T
• Y at position 1 of CDR2β
• L
• N at position 3 of CDR2β
• A
• E at position 5 of CDR2β
• Y
• I at position 5 of CDR3β
• V or L
• A at position 8 of CDR3β
• S or G
• G at position 9 of CDR3β
• E or N or D.
• the α chain variable domain of the TCR comprises an amino acid sequence having at least 90% sequence homology with the amino acid sequence shown in SEQ ID NO:
• 1, and/or the β chain variable domain of the TCR comprises an amino acid sequence having at least 90% sequence homology with the amino acid sequence shown in SEQ ID NO:
• 2.