Back to results
US 2022/0016592 A1Published application
Methods and compositions of localizing nucleic acids to arrays
AssigneeIllumina Cambridge Limited
InventorsMark Edward Brennan Smith, Andrea Sabot, Isabelle Marie Julia Rasolonjatovo, Jean-Ernest Sohna Sohna, Adrian Martin Horgan, Harold Philip Swerdlow
Life of this patent
Estimating the term…
Abstract
Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.
Claims21
(canceled)
A method of preparing an array of nucleic acids, comprising:
- immobilizing a population of polynucleotides on a surface of a solid support, wherein the population of polynucleotides comprises a first plurality of polynucleotides, wherein each polynucleotide of the first plurality of polynucleotides comprises the nucleotide sequence set forth in SEQ ID NO:03 or 04.
A method of sequencing a population of template nucleic acids, comprising:
- (a) hybridizing the population of template nucleic acids to a population of polynucleotides immobilized on a surface of a solid support, wherein the population of polynucleotides comprises a first plurality of polynucleotides, wherein each polynucleotide of the first plurality of polynucleotides comprises the nucleotide sequence set forth in SEQ ID NO:03 or 04;
- (b) extending the population of polynucleotides hybridized to the population of template nucleic acids;
- and
- (c) sequencing the extended population of polynucleotides.
1 more element
Dependencies are inferred from the claim text.