Compositions and methods for assessing eye vasculature

A method of assessing the location of a disease or an injury in a subject's eye vasculature, comprising the steps of:

• a. administering an effective amount of a compound of structural Formula I
• or a salt thereof;
• wherein
• X1 and X2 are independently chosen from —CO(AA), —CN, —CO2R1, —CONR2R3, —COR4, —NO2, —SOR35, —SO2R6, —SO2OR7 and —PO3R8R9;
• wherein at least one of X1 and X2 is —CO(AA);
• Y1 and Y2 are independently chosen from —OR10, —SR11, —NR12R13, —N(R14)COR15, —CONH(PS);
• —P(R16)2, —P(OR17)2 and
• each Z1 is independently chosen from a bond, —CR18R19—, —O—, —NR20—, —NCOR21—, —S—, —SO—, and —SO2—;
• each R1 to R21 are independently chosen from hydrogen, C1-C10 alkyl, C1-C10 alkyl substituted with hydroxyl and carboxylic acid, C3-C6 polyhydroxylated alkyl, C5-C10 aryl, C5-C10 heteroaryl, C3-C5 heterocycloalkyl, C3-C5 heterocycloalkyl substituted with C(O), —(CH2)aCO2H, —(CH2)aCO2H substituted with C5-10 heteroaryl, —(CH2)aCONR30R31, —(CH2)aNHSO3−, —(CH2)aNHSO3H, —(CH2)aOH, —(CH2)aOPO3═, —(CH2)aOPO3H2, —(CH2)aOPO3H−, (CH2)aOR22, —(CH2)aOSO3−, —(CH)aOSO3H, —(CH2)aPO3═, —(CH2)aPO3H2, —(CH2)aPO3H−, —(CH2)aSO3, —(CH2)aSO3H, —(CH2)dCO(CH2CH2O)cR23, —(CH2)d(CH2CH2O)cR24, —(CHCO2H)aCO2H, —CH2(CHNH2)aCH2NR25R26, —CH2(CHOH)aCO2H, —(CH2(CHOH)aR27, —CH[(CH2)bNH2]aCH2OH, —CH[(CH2)bNH2]aCO2H, and —(CH2)aNR28R29;
• each R22 to R31 are independently chosen from hydrogen, C1-C10 alkyl, and C1-C5-dicarboxylic acid;
• R35 is chosen from C1-C10 alkyl, C1-C10 alkyl substituted with hydroxyl and carboxylic acid, C3-C6 polyhydroxylated alkyl, C5-C10 aryl, C5-C10 heteroaryl, C3-C5 heterocycloalkyl, C3-C5 heterocycloalkyl substituted with C(O), —(CH2)aCO2H, —(CH2)aCO2H substituted with C5-C10 heteroaryl, —(CH2)aCONR30R31, —(CH2)aNHSO3−, —(CH2)aNHSO3H, —(CH2)aOH, —(CH2)aOPO3−, —(CH2)aOPO3H2, —(CH2)aOPO3H−, —(CH2)aOR22, —(CH2)aOSO3−, —(CH2)aOSO3H, —(CH2)aPO3═, —(CH2)aPO3H2, —(CH2)aPO3H−, —(CH2)aSO3−, —(CH2)aSO3H, —(CH2)dCO(CH2CH2O)cR23, —(CH2)d(CH2CH2O)cR24, —(CHCO2H)aCO2H, —CH2(CHNH2)aCH2NR25R26, —CH2(CHOH)aCO2H, —CH2(CHOH)aR27, —CH[(CH2)bNH2]aCH2OH, —CH[(CH2)bNH2]aCO2H, and —(CH2)aNR25R29;
• (AA) is a polypeptide chain comprising one or more natural or unnatural amino acids linked together by peptide bonds;
• (PS) is a sulfated or non-sulfated polysaccharide chain comprising one or more monosaccharide units connected by glycosidic linkages;
• and
• each ‘a’, ‘b’, and ‘d’ are independently chosen from 0 to 10, ‘c’ is chosen from 1 to 100 and each of ‘m’ and ‘n’ independently is an integer from 1 to 3;
• and;
• b. irradiating the subject's eye vasculature with non-ionizing radiation, wherein the radiation causes the compound to fluoresce;
• c. detecting the fluorescence of the compound in the subject's eye vasculature;
• and
• d. assessing the location of disease or injury in the subject's eye vasculature, based on the detected fluorescence.