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US 2022/0016177 A1Published application
Universal donor cells
AssigneeCrispr Therapeutics AG
InventorsAlireza REZANIA, Rebeca RAMOS-ZAYAS
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AssigneeCrispr Therapeutics AG
InventorsAlireza REZANIA, Rebeca RAMOS-ZAYAS
Estimating the term…
Genetically modified cells that are compatible with multiple subjects, e.g., universal donor cells, and methods of generating said genetic modified cells are provided herein. The universal donor cells comprise at least one genetic modification within or near at least one gene that encodes a survival factor, wherein the genetic modification comprises an insertion of a polynucleotide encoding a tolerogenic factor. The universal donor cells may further comprise at least one genetic modification within or near a gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or a component or a transcriptional regulator of a MHC-I or MHC-II complex, wherein said genetic modification comprises an insertion of a polynucleotide encoding a second tolerogenic factor.
A genetically modified cell comprising a nucleotide sequence encoding HLA class I histocompatibility antigen, alpha chain E (HLA-E) inserted within a thioredoxin interacting protein (TXNIP) gene locus, the nucleotide sequence inserted in the TXNIP gene locus comprising SEQ ID NO:
Dependencies are inferred from the claim text.