Non-enzymatic glow assays

Abstract

The present disclosure relates to improved light-based assays, especially ligand binding assays, in which light is emitted from moieties stimulated by stimulating moieties. There is provided a method of generating and detecting a light signal comprising (a) providing a fluid sample comprising an analyte of interest; (b) contacting said sample with a first reagent comprising a molecular recognition element that binds to said analyte; (c) contacting said sample with a second reagent that (i) binds to said analyte in the presence of said first reagent; or (ii) binds to said first reagent; wherein said second reagent contains a light-generating reporter or chemiexcitation emitter; (d) removing unbound first and second reagents; (e) introducing into said sample a source of singlet oxygen or a chemiexcitation stimulator; and (f) measuring the production of light from said reporter.

Dependencies are inferred from the claim text.