Rapid diagnostic test
AssigneeDetect, Inc.
InventorsHenry Kemble, Spencer Glantz, Jeffrey Sabina, Sebastian Jaramillo-Riveri
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Abstract
Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent detection of the amplification products.
Claims20
An oligonucleotide strand displacement probe comprising:
- (i) a first single stranded polynucleotide having a 5′ portion and a 3′ portion, wherein the 3′ portion comprises a first hapten or the 5′ portion comprises a first hapten and the 3′ portion comprises a first extension blocking group;
- and
- (ii) a second single stranded polynucleotide comprising a 5′ portion and a 3′ portion, wherein the 3′ portion comprises a second hapten or the 5′ portion comprises a second hapten and the 3′ portion comprises a second extension blocking group,
- wherein the second single stranded polynucleotide is hybridized to the first single stranded polynucleotide to form a duplex region and a single stranded toehold region comprising the 3′ portion of the first single stranded polynucleotide.
A mediator displacement probe comprising a hemi-duplex nucleic acid comprising:
- (i) a first single stranded polynucleotide having a 5′ end and a 3′ end, comprising a target sequence-specific portion contiguously linked to a mediator complement (MedC) portion;
- and
- (ii) a second single stranded polynucleotide having a 5′ end and a 3′ end, comprising a mediator (Med) polynucleotide sequence, a label attached to the 5′ end of the Med polynucleotide sequence, and an extension blocking agent attached to the 3′ end of the Med polynucleotide sequence,
- wherein the MedC portion of the first single stranded polynucleotide and the Med polynucleotide sequence of the second single stranded polynucleotide are annealed together to form a hemi-duplex molecule, wherein the target sequence-specific portion of the first single stranded polynucleotide forms the single stranded portion of the hemi-duplex molecule.
Dependencies are inferred from the claim text.