Molecular assessment of trbc usage
AssigneeAutolus Limited
InventorsShimobi Onuoha, Meggan Czapiga, Biao Ma
Life of this patent
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Abstract
The invention relates to a method of determining the T cell receptor β chain (TRBC) gene type of a cell, the method comprising (a) determining the J gene type expressed in said cell, and (b) inferring from (a) the TRBC gene type expressed in said cell. The invention further relates to use of a CAR T cell targeted to a T cell receptor β chain (TRBC) type 1, or a CAR T cell targeted to a T cell receptor β chain (TRBC) type 2. The invention further relates to a method of medical treatment, and to nucleic acid probes.
Claims21
A method of determining the T cell receptor β chain (TRBC) gene type of a cell, the method comprising
- (a) determining the J gene type expressed in said cell, and
- (b) inferring from (a) the TRBC gene type expressed in said cell.
(canceled)
A nucleic acid probe comprising a nucleotide sequence:
- SEQ ID NO:
- 1
- tgaacactgaagctttctttggacaaggcaccaga
- ctcacagttgtag,
- SEQ ID NO:
- 2
- ctaactatggctac
- accttcggttcggggaccaggttaaccgttgtag,
- SEQ ID NO:
- 3
- ctctggaaacaccatatatttttttttggcagtgga
- acccagctctctgtcttgg,
- SEQ ID NO:
- 4
- caactaatgaaaaactgttttttggcagtggaacc
- cagctctctgtcttgg,
- SEQ ID NO:
- 5
- tagcaatcagccccagcattttggtgatgg
- gactcgactctccatcctag,
- SEQ ID NO:
- 6
- ctcctataattcacccctccactttgggaatgggacca
- ggctcactgtgacag,
- SEQ ID NO:
- 7
- ctcctataatt
- cacccctccactttgggaacgggaccaggct
- cactgtgacag,
- SEQ ID NO:
- 8
- ctcctacaatgagcagttcttcgggccagggac
- acggctcaccgtgctag,
- SEQ ID NO:
- 9
- cgaacaccggggagctgttttttggagaaggctct
- aggctgaccgtactgg,
- SEQ ID NO:
- 10
- ctgagaggcgctgctgggcgtctgggcggagga
- ctcctggttctgg,
- SEQ ID NO:
- 11
- agcacagatacgcagtattttggcccaggcacccgg
- ctgacagtgctcg,
- SEQ ID NO:
- 12
- agccaaaaacattcagtacttcggcgccgggacccgg
- ctctcagtgctgg,
- SEQ ID NO:
- 13
- accaagagacccagtacttcgggccaggcac
- gcggctcctggtgctcg,
- SEQ ID NO:
- 14
- ctctggggccaacgtcctgactttcggggccggcag
- caggctgaccgtgctgg,
- SEQ ID NO:
- 15
- ctcctacgagcagtacttcgggccgggcacc
- aggctcacggtcacag
- or
- SEQ ID NO:
- 16
- ctcctacgagcagtacgtcgggccgggcaccag
- gctcacggtcacag,
Dependencies are inferred from the claim text.