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US 2022/0010368 A1Published application
Enhanced detection of low-copy-number nucleic acids in an integrated workflow
OwnerMax Haesendonckxinventor
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Abstract
The present invention concerns methods and strategies of detecting low-copy-number nucleic acids in integrated work-flows on automated or semi-automated platforms, using a pre-amplification reaction positioned to only a portion of a silica surface in an extraction chamber of the platform. The present methods of the invention open the possibility to expand the repertoire of already developed automated workflows to enable them to process even very diluted samples such as bodily fluids, including liquid biopsies.
Claims15
1-15. (canceled)
A method of detecting a low copy number nucleic acid in an automated system, the method comprising:
- contacting a biological sample with a hydroxyapatite (HAP) solid support to obtain a sample comprising a low copy number nucleic acid;
- providing the sample comprising the low copy number nucleic acid to an extraction chamber of the automated system, said extraction chamber comprising silica surface for DNA adsorption;
- adsorbing the nucleic acid to the silica surface;
- and
- amplifying the nucleic acid within the automated system.
2 more elements
Dependencies are inferred from the claim text.